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KMID : 1094720220270030386
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Biotechnology and Bioprocess Engineering
2022 Volume.27 No. 3 p.386 ~ p.397
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The Inhibitory Effect of Corni Fructus against Oxidative Stress-induced Cellular Damage in C2C12 Murine Myoblasts
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Kim Sung-Ok
Jeung Ji-Suk
Park Cheol
Lee Hye-Sook
Choi Sung-Hyun
Kim Gi-Young
Kim Hye-Young
Choi Yung-Hyun
Lee Eun-Joo H.
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Abstract
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Although the beneficial biological effects of Cornus officinalis fruit, Corni Fructus, are known to be correlated with antioxidant efficacy, the protective effect of muscle cells against oxidative damage is still unclear. In this study, we aimed to investigate the potential protective effect of Corni Fructus water extract (CFE) against cellular damage caused by oxidative stress in C2C12 murine myoblast cells. The results show that, in the non-cytotoxic range, CFE increased the expression of nuclear factor-erythroid-2 related factor 2 (Nrf2) and its phosphorylation. CFE also up-regulated heme oxygenase-1 (HO-1) expression, one of the representative downstream factors of Nrf2, indicating CFE activated the Nrf2/HO-1 signaling pathway. In addition, CFE pretreatment significantly ameliorated growth reduction and suppressed DNA damage and apoptosis in hydrogen peroxide (H2O2)-treated C2C12 myoblast, which was correlated with inhibition of reactive oxygen species generation. Furthermore, H2O2-induced mitochondrial dysfunction linked to apoptosis by increasing levels of cytoplasmic cytochrome c and Bax/Bcl-2 was restored by CFE in C2C12 cells. CFE also suppressed H2O2-induced activation of caspase-9 and -3 and cleavage of poly (ADP-ribose) polymerase. However, zinc protoporphyrin, a selective inhibitor of HO-1, markedly abolished the preventive effect of CFE, demonstrating that CFE protected C2C12 myoblasts from H2O2-induced damage via activation of the Nrf2/HO-1 antioxidant axis, and that CFE may be applicable as a therapeutic agent for oxidative stress-mediated muscle damage.
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KEYWORD
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Corni Fructus, DNA damage, apoptosis, reactive oxygen species
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